Trish Berger, Ph.D.

Position Title

Distinguished Professor

2147 Meyer Hall

Education

B.A., Biochemistry, University of Kansas
(Year Abroad) Physiology and Biochemistry of Farm Animals, University of Reading, England
M.S., Animal Science, Purdue University
Ph.D., Animal Science, Purdue University

Research Interests

A long-term focus in our laboratory is mammalian fertilization and the molecules involved in the fertilization process. Research is centered on interactions between sperm and oocyte plasma membrane molecules after sperm have undergone the acrosome reaction since our earlier work demonstrated that this was frequently the limiting step in vivo in subfertile animals. Details of these interactions including receptor/ligand pairings are still largely unknown. Environmental stresses such as elevated ambient temperature affect both the sperm’s ability and the oocyte’s ability to interact with each other at the plasma membrane level. We hypothesize that this is due to decreased/defective synthesis of interacting molecules.

A second focus in our laboratory is the regulation of Sertoli cell proliferation. Sertoli cell numbers are a major determinant of postpuberal testis size and sperm production. The number of Sertoli cells is generally believed to be determined prepuberally. We have found that reducing endogenous estrogen in the boars postnatally leads to increased proliferation of Sertoli cells and larger postpuberal testes in the pig. This occurs without altered pituitary hormones in boars and strongly suggests a locally mediated mechanism.

The pig is the primary species studied due in part to its importance to US and world food production as well as the availability of large numbers of gametes and comparatively short gestation and prepuberal intervals. In vitro oocyte maturation and fertilization, in vitro bioassays, gene expression (qPCR), immunohistochemistry, gel electrophoresis and western blotting, heterospermic insemination and other in vivo animal trials, CRISPR editing and embryo transfer are techniques used to advance our laboratory research goals.

Graduate Groups

Animal Biology

Courses Taught

Physiology of Reproduction (NPB 121/121L)
Mammalian Gametogenesis and Fertilization (MCP 222)
Stress and Reproduction (ANS 160A&B)

People

Research Staff

Barbara Jean S. Nitta

Recent Graduate Students

Tana Almand, M.S.
Jennifer Jankovitz, M.S.
Kimberley Miller, M.S.
Simin Tang, M.S.
Javier Morales, Ph.D.
Kelly Zacanti, Ph.D.
Maria Carolina Zimara M.S.

Selected Publications

Berger T, Guerrero V, Boeldt R, Legacki E, Roberts M, and Conley AJ. 2024. Development of porcine accessory sex glands. Animals, 14(3): 462.

Park I, Navarro-Serna S, Pinho R, Berger T, Maga EA, Gadea J, Kim SK, Ross PJ. 2024. Electroporation of CRISPR/Cas9 targeting neurogenin 3 (NGN3) in porcine embryos and its effects on mosaicism and off-target effects by next generation sequencing (NGS). Re: GEN Open, 4: 9-20.

Noto V, Nitta-Oda BJ, Berger T. 2024. Gene expression in porcine bulbourethral glands. Animals, 14(7): 1115.

Zacanti K, Park I, McNabb BR, Urbano TM, Maga EA, Rowe JD, Hennig SL, Ross P, and Berger T. 2023. Gender disparity in survival of early porcine fetuses due to altered androgen receptor or associated U2 spliceosome component. Scientific Reports, 13: 15072.

Kang S and Berger T. 2023. Macrophages in juvenile pig testis: Link with increase in Sertoli cells induced by estradiol suppression. Reproduction in Domestic Animals, 58: 564-568.

Berger T, Vanselow J, Conley A, Almand TJ, and Nitta-Oda BS. 2022. Multifaceted Epigenetic Regulation of Porcine Testicular Aromatase. Molecular and Cellular Endocrinology, 541: 111526.

Conley AJ, Berger T, Arias del Razo R, Cotterman RF, Sahagún E, Goetze LR, Jacob S, Weinstein TAR, Dufek ME,Mendoza SP, and Bales KL. 2022.The Onset of Puberty in Colony- housed Male and Female Titi Monkeys (Plecturocebus cupreus): Possible Effects of Oxytocin Treatment during Peri-adolescent Development. Hormones and Behavior, 142(105157).

Katleba K, Legacki E, and Berger T. 2022. Expression of CSF1, AR, and SRD5A2 during postnatal development of the boar reproductive tract. Animals, 12: 2167.

Berger, T., Tang, S., Tu, L., Soto, D.A., Conley, A.J. and Nitta-Oda, B., 2021. Changes in testicular gene expression following reduced estradiol synthesis: A complex pathway to increased porcine Sertoli cell proliferation, Mol Cell Endocrinol. 523, 111099.

Vanselow, J., Conley, A.J. and Berger, T., 2021. Aromatase and the three little paralogs, Biol Reprod. https://academic.oup.com/biolreprod/advance-article-abstract/doi/10.1093/biolre/ioab112/6294498

Vanselow, J., Conley, A.J., Corbin, C.J. and Berger, T., 2021. Genomic Structure of the Porcine CYP19 Locus and Expression of the CYP19A3 Paralog, Genes (Basel). 12. https://www.mdpi.com/2073-4425/12/4/533

Berger T, Nitta-Oda BJ. 2020. Sertoli cell proliferation in juvenile boars and microRNA. Livestock Science 233:103954.

Berger, T, Sidhu P, Tang S, Kucera H. 2019. Are testicular cortisol and WISP2 involved in estrogen-regulated Sertoli cell proliferation? Anim Reprod Sci. 207: 44-51. doi: 10.1016/j.anireprosci.2019.05.014

Kucera H, Puschner B, Conley A, Berger T. 2019. Tissue steroid levels in response to reduced testicular estrogen synthesis in the male pig, Sus scrofa. PLOS One 14(4):e3900215. doi: 10.1371/journal.pone.0215390

Berger T. 2019. Testicular estradiol and the pattern of Sertoli cell proliferation in prepuberalbulls. Theriogenology 136:60-65. doi:10.1016/j.theriogenology.2019.06.031

Hughes JR, Berger T. Regulation of apical blebbing in the porcine epididymis. J Anat. 2018; 232:515-522.

Berger T, Nitta-Oda BJ. 2018. Increased testicular estradiol during the neonatal interval reduces Sertoli cell numbers. Anim Reprod Sci. 189:146-151.

Legacki E, Conley AJ, Nitta-Oda BJ, Berger T. Porcine Sertoli Cell Proliferation after Androgen Receptor Inactivation. Biology of Reproduction 2015; 92:93.

Katleba K, Legacki EL, Conley AJ, Berger T. Steroid regulation of early postnatal development in the corpus epididymidis of pigs. Journal of Endocrinology 2015; http://dx.doi.org/10.1530/JOE-15-0001.

Tanphaichitr N, Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, Souda P, Angel JB, Faull KF, Aitken RJ, Whitelegge J, Hardy D, Berger T, Baker M. Remodeling of the plasma membrane in preparation for sperm-egg recognition: roles of acrosomal proteins. Asian Journal of Andrology 2015; 17(4): 1-9.

Hughes JR, Berger T. Development of apical blebbing in the boar epididymis. PLOS One 2015; 10(5):e0126848, 2015.

Berger T, Conley AJ. Reduced endogenous estrogen and hemicastration interact synergistically to increase porcine Sertoli cell proliferation. Biology of Reproduction 2014.

Berger T, Conley AJ. Reducing endogenous estrogen during prepuberal life does not affect boar libido or sperm fertilizing potential. Theriogenology 2014, 82: 627-35.

Zamaratskaia G, Berger T. Skatole metabolism in the pigs with reduced testicular oestrogen synthesis. Reproduction in Domestic Animals 2014; 49(2): 302-305.

Berger T, Conley AJ, Van Klompenberg M, Roser JF, Hovey RC. Increased testicular Sertoli cell population induced by an estrogen receptor antagonist. Molecular and Cellular Endocrinology 2013; 366: 53-58.

Kao E, Villalon R, Ribeiro S, Berger T. Role for endogenous estrogen in prepubertal Sertoli cell maturation. Anim Reprod Sci 2012; 135:106-112.

Berger T, Kentfield L, Roser JF, Conley A. Stimulation of Sertoli cell proliferation: defining the response interval to an inhibitor of estrogen synthesis in the boar. Reproduction 2012; 143: 523-529.

Conley AJ, Corbin CJ, Thomas JL, Gee NA, Lasley BL, Moeller BC, Stanley SD, Berger T. Costs and consequences of cellular compartmentalization and substrate competition among human enzymes involved in androgen and estrogen synthesis. Biology of Reproduction 2012; 86: (1) 1-8.

Berger T, McCarthy M, Pearl CA, At-Taras E, Roser JF, Conley A. Reducing endogenous estrogens during the neonatal and juvenile periods affects reproductive tract development and sperm production in postpuberal boars. Anim Reprod Sci 2007.

At-Taras EE, Berger T, McCarthy MJ, Conley AJ, Nitta-Oda BJ, Roser JF. Reducing estrogen synthesis in developing boars increases testis size and total sperm production. J Androl 2006; 27: 552-559.